Angiogenesis

Xenograft-CAM assay control

Xenograft-CAM assay with treatment

The Southern Research Angiogenesis Program provides an integrated series of in vitro and in vivo assays and analyses to screen and evaluate compounds and biological agents affecting the angiogenic process.

In vitro assays

• Endothelial cell proliferation
• Cell migration / invasion
• Endothelial tube formation
• Endothelial cell apoptosis
• Assays using tumor and endothelial cell co-cultures
• Angiogenesis assays for High Throughput Screening (HTS)

In vivo assays and analyses

• Chick Chorioallantoic Membrane (CAM) assay with quantitative analysis of blood vessel formation
• Tumor angiogenesis Xenograft-CAM assay where human tumor cells are implanted on CAM, and blood vessel formation are quantitatively analyzed
• Matrigel Plug assay in mice with quantitative analysis of microvessel density (MVD)
• Analysis of angiogenic effect for Xenograft mouse study, including tumor MVD and levels of angiogenic growth factors in blood serum and tumor samples
• A new xenograft mouse model with regulated tumor angiogenesis and inducible metastatic phenotype, used in preclinical efficacy studies to test potential anti-cancer drugs and to evaluate new treatment modifications

In recent years, the Angiogenesis Program at Southern Research has evaluated several hundred compounds as anti- or pro-angiogenic agents for a variety of clients. We are flexible in assay and experiment design based on the specific objectives of each study.